Insights at the intersection of glycobiology and biotechnology
Explore research perspectives, analytical workflows and applied studies from GlycoSeLect’s work with Recombinant Prokaryotic Lectins.
Latest from the laboratory
Bio-layer interferometryBio-Layer Interferometry with Recombinant Prokaryotic Lectins
Explains Bio-Layer Interferometry (BLI), how optical biosensors measure binding in real time, and how GlycoSeLect's recombinant prokaryotic lectins (RPLs) can be used on BLI platforms for faster, higher-throughput glycoanalysis.
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A curated collection covering RPL-enabled analysis, purification and emerging applications.
Glycoprotein analysisRecombinant Prokaryotic Lectins offer improved glycoprotein analysis and purification
Discusses why glycosylation is a critical quality attribute in biologics and compares recombinant prokaryotic lectins with traditional plant-derived lectins. It highlights stronger binding performance, scalability and the possibility of engineering RPLs for specific glycan targets.
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BiopharmaceuticalsDevelopment of Simple, Rapid & High-Throughput Glycoanalytics for Biopharmaceuticals
Describes a project integrating GlycoSeLect RPLs with the Sartorius Octet BLI platform to develop a faster glycoanalysis workflow for biopharmaceuticals. The article covers method design, linearity, repeatability and spike-recovery testing.
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GlycoanalysisSimplifying Glycoanalysis with Recombinant Prokaryotic Lectins
Explains limitations of conventional HPLC- and mass-spectrometry-based glycoanalysis and presents RPL biosensors on BLI systems as a rapid, label-free and potentially higher-throughput alternative for monitoring glycosylation.
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GlycopurificationStreamline Purification of Glycosylated Proteins with Recombinant Prokaryotic Lectins
Focuses on glycoprotein purification. It contrasts conventional chromatographic approaches with RPL affinity resins and describes work purifying HIV gp120 envelope glycoproteins with galactose- and mannose-specific RPL matrices.
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Industrial applicationsTargeting key glycoproteins: Glucose oxidase
Introduces glucose oxidase as a glycoprotein target and links it to GlycoSeLect's mannose-specific RPLs. It reviews applications of glucose oxidase in glucose sensing, biofuel cells, food and beverage processing, wine production, oral care and gluconic-acid production.
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Disease biologyGlycoproteins and disease
Reviews the role of glycosylation and glycoproteins in disease biology, including autoimmune conditions, cancer, HIV and COVID-19. It then connects glycan profiling and GlycoSeLect RPLs with analytical and research applications.
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CollaborationsCollaborating on new tools for glycosylation analysis
Describes a collaboration between GlycoSeLect and IonDx to combine RPLs with ion mobility spectrometry for rapid glycosylation analysis of biotherapeutics, including monoclonal antibodies.
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Coronavirus researchUse of Recombinant Prokaryotic Lectins (RPLs) for Coronavirus Research
Examines the role of glycosylation on coronavirus spike proteins and explains why lectins may be useful research tools for studying viral glycoproteins, analytics and purification. The article was written early in the COVID-19 pandemic and should be treated as historical scientific content.
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BiopharmaceuticalsRPLs for high-throughput analysis of biopharmaceuticals
Presents a project in which GlycoSeLect RPLs were integrated into a biosensor platform to evaluate glycosylation in biopharmaceutical samples. It describes work with FSH and Eylea and reports assay repeatability, linearity and spike-recovery testing.
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